TABLE 1.

Antibodies used in this study

AntibodyHost animalDilutionCompanya
Annexin V-FITCMouse (apoptosis marker)1:100BD Pharmingen
Anti-CD11c (N418)Mouse (DC marker)1:100BD Pharmingen
CD11c (HL3)-FITCMouse (DC marker)1:100BD Pharmingen
CD11c (HL3)-PEMouse (DC marker)1:100BD Pharmingen
CD4 (L3T4)-FITCMouse (T-cell marker)1:100BD Pharmingen
CD40 (1C10)-PEMouse (DC activation marker)1:100BD Pharmingen
CD80 (16-10A1)-PEMouse (DC activation marker)1:100BD Pharmingen
CD86 (GL1)-PEMouse (DC activation marker)1:100BD Pharmingen
IAb β-chain (AF-120.1)-PEMouse (DC activation marker)1:100BD Pharmingen
IL-10 (JESS-16E3)-FITCMouse (Th2 marker)1:100BD Pharmingen
IL-12p40/p70 (C15.6)-PEMouse (Th1 marker)1:100BD Pharmingen
H2Kb (AF6-88.5)-PEMouse (DC activation marker)1:100BD Pharmingen
p-ERK1/2Mouse (signal marker)1:1,000Cell Signaling
ERK1/2Mouse (signal marker)1:2,000Cell Signaling
p-p38Mouse (signal marker)1:1,000Cell Signaling
p38Mouse (signal marker)1:2,000Cell Signaling
Topo I (C-15)Mouse (nucleus protein marker)1:2,000Santa Cruz
p-65Mouse (signal marker)1:500Abcam
  • a BD Pharmingen, BD Pharmingen, San Jose, CA; Cell Signaling, Cell Signaling, Danvers, MA; Santa Cruz, Santa Cruz Biotechnology, Santa Cruz, CA; Abcam, Abcam, Cambridge, MA.