TABLE 2.

Primers used in this study

PrimerSequencea
Sp0090.1GCTCTAGACATTGAGAGAGACAACTGG
Sp0090.2CGCTCTAGACAAGAAGTAAGGGAAC
Sp0149.1GCTCTAGAGGCTCTTGCAGCTTGCGG
Sp0149.2CGCTCTAGAGGCTTTCGTTTGTAGCGTC
Sp0610.1GCTCTAGATTACGGAGACTACCACG
Sp0610.2CGCTCTAGATCCACCAGATAGCATGCC
Sp0710.1GCTCTAGATTGGGCGTTACGATTG
Sp0710.2CGCTCTAGAGTGCCTGTCCACTTTC
Sp0750.1GCTCTAGACGCGCTGTTAGCCCTAGG
Sp0750.2GCTCTAGATGATACTGCACGCATGGC
Sp846.1GCTCTAGATGTTAGCAGGCCTTCTTG
Sp846.2CGCTCTAGAGCCCCTGCAATTTCAAC
Sp1690.1GCTCTAGATTGCGCTAGCGGCTGTTG
Sp1690.2GCTCTAGAGCTTCCTCCACCACTACG
Sp1798.1GCTCTAGATGACTGTCCCCGGTTTAG
Sp1798.2CGCTCTAGATTGTTGATTGGTCCTCCC
Sp1826.1GCTCTAGACGACTGCTTCTTCATCTG
Sp1826.2CGCTCTAGAATTGCCCGTCCTGTACC
Sp2084.1GCTCTAGATTTGGGCTTGTTGCCTG
Sp2084.2CGCTCTAGATGTGACCACTTGTTGACC
Sp2108.1GCTCTAGAACTGCTACACTTGCTAG
Sp2108.2CGCTCTAGAACCAAGGCTACCTAC
Cm.1TTATAAAAGCCAGTCATTAG
Cm.2TTTGATTTTTAATGGATAATG
Sp1TCGAGATCTATCGATGCA
Sp3GGATCCATATGACGTCGA
EryFATGAACAAAAATATAAAATA
EryRTTATTTCCTCCCGTTAAATAAT
Sp0749FCACTGACAATGCCAGTGACTATGC
Ery-Sp0749RTATTTTATATTTTTGTTCATAAGATTCACTCTTTCTATTTATAA
Ery-Sp0753FATTATTTAACGGGAGGAAATAAAACATTCCAGTGGATTGTTTTAG
Sp0754RGCGGAATATTGACTGTATGGGAG
Sp0749FwdCGGGATCCTGTGGAGAAGTGAAGTCTGGA
Sp0749RvCGGGATCCTTATGGTTTTACAACTTCTGC
Sp0749RT1CGATGCAGACCACAACAC
Sp750RT2CCTAGGGCTAACAGCGC
750RT1.1GATGGGGGTTACTCCAGG
751RT2.1CCCAGAGTTGCTACCGC
751RT1.1GGTGCGATTGTTTCGG
752RT2.1GGTTCCGTAGCAAGGG
Sp0752RT1GGCCGTTTAATCGCTCAAG
Sp0753RT2GGGCGCGTCCCATGGCAAG
753RT1.1GGAGAATCGTCCTATCAG
754RT2.1CAGGCAGACGGTGCAAACC
  • a Restriction enzyme sites in 5′ linkers are underlined.