TABLE 1.

Strains and plasmids constructed and/or used in this study

Plasmid or strainDescription (source or reference)a
Plasmids
    pID701Shuttle vector for IDM transformation of S. pneumoniae: Cmr (22)
    pPC110pID701 containing an internal portion of Sp0090 in the XbaI site: Cmr (this study)
    pPC111pID701 containing an internal portion of Sp0149 in the XbaI site: Cmr (this study)
    pPC112pID701 containing an internal portion of Sp0610 in the XbaI site: Cmr (this study)
    pPC113pID701 containing an internal portion of Sp0710 in the XbaI site: Cmr (this study)
    pPC114pID701 containing an internal portion of Sp1796 in the XbaI site: Cmr (this study)
    pPC115pID701 containing an internal portion of Sp1824 in the XbaI site: Cmr (this study)
    pPC116pID701 containing an internal portion of Sp0750 in the XbaI site: Cmr (this study)
    pPC117pID701 containing an internal portion of Sp1690 in the XbaI site: Cmr (this study)
    pPC118pID701 containing an internal portion of Sp0846 in the XbaI site: Cmr (this study)
    pPC119pID701 containing an internal portion of Sp2084 in the XbaI site: Cmr (this study)
    pPC120pID701 containing an internal portion of Sp2108 in the XbaI site: Cmr (this study)
    pPC139pQE30 carrying full length livJ: Kmr Ampr (this study)
Strains
    0100993S. pneumoniae capsular serotype 3 clinical isolate (22)
    ΔSp00900100993 containing an insertion made with plasmid pPC110: Cmr (this study)
    ΔSp01490100993 containing an insertion made with plasmid pPC111: Cmr (this study)
    ΔSp06100100993 containing an insertion made with plasmid pPC112: Cmr (this study)
    ΔSp07500100993 containing an insertion made with plasmid pPC116: Cmr (this study)
    ΔSp08460100993 containing an insertion made with plasmid pPC118: Cmr (this study)
    ΔSp16900100993 containing an insertion made with plasmid pPC117: Cmr (this study)
    ΔSp17960100993 containing an insertion made with plasmid pPC114: Cmr (this study)
    ΔSp18240100993 containing an insertion made with plasmid pPC115: Cmr (this study)
    ΔSp20840100993 containing an insertion made with plasmid pPC119: Cmr (this study)
    ΔSp21080100993 containing an insertion made with plasmid pPC120: Cmr (this study)
    ΔlivHMGF0100993 containing the livHMGF deletion construct: Ermr (this study)
    T4ΔlivHMGFTIGR4 containing the livHMGF deletion construct: Ermr (this study)
  • a Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Ermr, erythromycin resistance.