TABLE 2.

Proteins observed in leptospiral OM fractions by 1D and/or 2D gel electrophoresis at concentrations equal to or greater than the concentrations found in whole cellsa

ProteinIdentityAntigenicity
p116
p90
p48LipL48
p44b+
p41LipL41+
p36aLipL36
p36b
p32LipL32+
p31aP31LipL45±
p31b
p31c
p22+
p21.5b
p21bLipL21c
  • a The identities of known OMPs are shown in the middle column. Comparisons with 1D and 2D immunoblots using clinical leptospirosis sera allowed the identification of some of these proteins as antigens recognized by the humoral immune response during human leptospirosis (14), as indicated in the right-hand column.

  • b Protein for which peptide sequencing has been performed in a recently reported study of L. interrogans serovar Lai OMPs (9).

  • c The pL21 protein has been identified as an OM lipoprotein designated LipL21 (P. A. Cullen, B. Adler, D. M. Bulach, D. A. Haake, and R. L. Zuerner, unpublished data).