TABLE 1.

Principal bacterial strains and plasmids used in this study

Bacterium or plasmidGenotype or relevant featuresReference
Bacteria
    INH34ΔfurA-katG strain of M. tuberculosis 30
    H37RvGenome sequenced strain of M. tuberculosis 5
Plasmids
    pKATII E. coli expression vector containing katG of M. tuberculosis 33
    pK275PpKATII with T275P mutation in katG 33
    pK139VpKATII with A139V mutation in katGThis study
    pK315TpKATII with S315T mutation in katG 33
    pKINTMycobacterial integration vector 30
    pPD28pKINT with 4.8-kb fragment spanning the furA-katG locus of M. tuberculosis 30
    pAP01pPD28 with deletional interruption of katG 30
    pAP21pPD28 with T275P mutation in katGThis study
    pAP22pPD28 with A139V mutation in katGThis study
    pAP23pPD28 with S315T mutation in katGThis study