TABLE 2.

T-cell responses of immunized mice the day before challenge with the C. trachomatis MoPn straina

AntigenAdjuvantImmunizationT-cell proliferative response (103 cpm) to:In vitro cytokine production (pg/ml)
IFN-γIL-4
RouteNo. of timesEBMediumEBMediumEBMedium
MOMPCpG + alumi.m. + s.c.16.5 ± 1.8b0.09 ± 0.01555 ± 31b<301.4 ± 0.7<0.1
OvalbuminCpG + alumi.m. + s.c.10.3 ± 0.20.1 ± 0.02<30<30<0.1<0.1
C. trachomatisi.n.16.9 ± 1.1b0.1 ± 0.044,275 ± 28b<305.1 ± 0.2b<0.1
MOMPCpG + alumi.m. + s.c.326.1 ± 4.1b1.5 ± 0.21,306 ± 85b<304.4 ± 1.3<0.1
OvalbuminCpG + alumi.m. + s.c.30.4 ± 0.10.3 ± 0.0839 ± 24<300.5 ± 0.7<0.1
C. trachomatisi.n.16.1 ± 0.8b0.2 ± 0.14,009 ± 137b<305.1 ± 1.2b<0.1
  • a The values are means ± standard errors for triplicate cultures. The data are from one of the experiments representative of duplicate experiments. UV-inactivated C. trachomatis MoPn EB were added at a ratio of EB to T cells of 10:1.

  • b P < 0.05 as determined by the Student t test for the comparison with the corresponding ovalbumin-immunized group.