TABLE 1.

Strains and plasmids

Strain or plasmidRelevant antibiotic resistanceaCharacteristicsReference(s)
E. faecalis strains
    OG1RFRif100, Fus25srtA positive, srtC positive, atn positive45, 46
    OG1RFΔatn (SJH-952)Rif100, Fus25srtA positive, srtC positive, atn negativeThis study
    OG1RFΔsrtA (SJH-953)Rif100, Fus25srtA negative, atn positiveThis study
    OG1RFΔsrtA srtA (SJH-954)Rif100, Fus25, Kan500srtA positive, atn positiveThis study
Plasmids
    pABG5Kan50 and Cm20 (Escherichia coli); Kan500 and Cm20 (E. faecalis)Shuttle vector containing rofA promoter for expression in gram-positive bacteria18
    pJRS233Erm750 (E. coli); Erm25 (E. faecalis)Temperature-sensitive plasmid for generation of in-frame deletions50
    pJRS233-ΔEF3056Erm750 (E. coli); Erm25 (E. faecalis)Temperature-sensitive plasmid for generation of in-frame deletion of EF3056 (srtA)29
    pJRS233-ΔatnErm750 (E. coli); Erm25 (E. faecalis)Temperature-sensitive plasmid for generation of in-frame deletion of EF0799 (atn)This study
    pAL1Kan50 (E. coli); Kan500 (E. faecalis)Derivative of pABG5, Cmr cassette, contains rofA promoter29
    pAL1::SrtAKan50 (E. coli); Kan500 (E. faecalis)Used for complementation of srtA deletion29
  • a The superscripts indicate the concentrations of antibiotics (in μg/ml) to which the strains and plasmids are resistant. Rif, rifampin; Fus, fusidic acid; Kan, kanamycin; Cm, chloramphenicol; Erm, erythromycin.