TABLE 1.

Fungal strains and plasmid constructs

Strain or plasmidDescriptionReference or source
Strains
    ATCC 46645 Aspergillus fumigatus wild-type strain 13
    AfS61 prtTΔ deletion mutant (prtT::loxP-Phleor-loxP)This study
    AfS62Reconstituted strain (prtT<ptrA>)This study
Plasmids
    pJET1.2Positive selection vector, pUC19 derivative (bla, multiple-cloning site in Eco47IR)Fermentas
    pSK275Plasmid containing ptrA cassette conferring resistance to pyrithiamine 27
    pSK341 Aspergillus marker cassette conferring resistance to phleomycin (pgpdA::ble/tk::trpCt) 26
    pSK462Replacement cassette for deletion of prtT in A. fumigatusThis study
    pSK463Reconstitution cassette containing prtT gene coupled to ptrA resistance cassetteThis study