TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsReference or source
E. coli
    EDL933Prototype EHEC O157:H730
    86-24EHEC O157:H7 strain 86-24; Smr Nalr38
    CVD46886-24; lpfA::cat Smr CmrThis study
    AGT300Derivative of wild-type EHEC O157:H7 strain 87-23 (stx mutant); SmrThis study
    AGT301AGT300; lpfA::cat Smr CmrThis study
    BL21(DE3)hsdS galcIts857ind1Sam7 nin5 lacUV5-T7 gene 1)35
    ORN172E. coli Δfim Kmr46
    SM10 (λ Pir)thi thr leuB tonA lacY supE recA::RP4-2-Tc::Mu-Km Kmr33
Plasmids
    pJOR5pACYC184 with 5,929-bp fragment containing lpfABCCDE; CmrThis study
    pSK+pBluescript cloning vector; AprStratagene
    plpfApBluescript SK(+) with KpnI/BamHI; lpfA AprThis study
    plpfA::catcat cassette in SmaI of lpfAThis study
    pBR322Cloning vector; Apr Cmr TcrNew England BioLabs
    pLPF100pBR322 with 5,929-bp BamHI; lpfABCCDE AprThis study
    pACYC184Cloning vector; source of Cmr cassette; Cmr TcrNew England BioLabs
    pRS551Protein fusion vector; Apr Kmr34
    pPLPFApRS551 with EcoRI/BamHI;1,164-bp lpf promoter regionThis study
    pT7-5T7 expression system vector; Apr36
    pLPFA01pT7-5 with EcoRI/BamHI;537-bp lpfA ORFThis study
    pCVD442Suicide vector; Apr Sucs7
    pLPF::catlpfA::cat in pCVD442This study