TABLE 1.

Oligonucleotides used in the present study

OligonucleotideSequenceDescription
NF1635′-TAAGCCATGGATAAGAAAAATATAG-3′Forward primer for SlpA precursor
NF1645′-AGGCTCGAGCATATCTAATAAA-3′Reverse primer for SlpA precursor and high-MW subunit
NF2385′-CACCACCACCACCACCACTGAC-3′Sense primer; hexahistidine encoding
NF2395′-TCGAGTCAGTGGTGGTGGTGGTGGTGAT-3′Antisense primer; hexahistidine encoding
NF2445′-TAAGCCATGGCAAATGATACAA-3′Forward primer for high-MW subunit
NF2455′-TAAGCCATGGCAACTACTGGAACA-3′Forward primer for low-MW subunit
NF2465′-AGGCTCGAGTGATTTAGTTTCTAATC-3′Reverse primer for low-MW subunit