TABLE 1.

Sequence of oligonucleotide primers used for amplification and cloning

GenePrimeraSequence (5′-3′)b
psaA21(F)AATCGTCATATGGCCATGGgcgctagcggaaaaaaagatgcagcttc
308(R)ATTCCCCTCGAGAAGCTTGGATCCtgccaatccttcagcaatctt
IL-221(F)AATCGTCATATGGCCATGGGCGGATCCgcacccacttcaagctccacttc
169(R)ATTCCCCTCGAGttgagggcttgttgagatgatgctttgaGagaaggctatcc
IL-422(F)AATCGTCATATGGCCATGGGCAAGCTTatccacggatgcgacaaaaatcac
140(R)ATTCCCCTCGAGcgagtaatccatttgcatgatgc
IL-1βSenseCATTTTAAGCTTgtacaaggagaaccaagcaacgacaaaatacctgtggcc
ttgggcgtacaaggagaaccaagcaacgacaaaGAATTCCTCGAGCACG
AntisenseCGTGCTCGAGGAATTCtttgtcgttgcttggttctccttgtacgcccaa
ggccacaggtattttgtcgttgcttggttctccttgtacAAGCTTAAAATG
  • a Numbers represent the first amino acid encoded by the forward (F) primers or the last amino acid encoded by the reverse (R) primers. All numbering is based on pre-pro amino acid sequences for each gene. Underlined nucleotides represent coding sequences for amino acids 163 to 171 of murine pre-pro IL-1β.

  • b Restriction sites in each primer relevant to this study are in bold; nucleotides in lowercase are derived from sequences of each gene deposited in GenBank.