TABLE 1.

Relative rates of hydrolysis of C4-HSL, C6-HSL, 3-oxo-C6, and C8 HSLs to open-chain forms at 22 and 37°Ca

AHLRelative rates of hydrolysis at:Rate at 37°C/ rate at 22°C
22°C37°C
C41304003.1
3-Oxo-C6701702.4
C6401102.8
C811.51.5
  • a The relative rates of hydrolysis were calculated as described in Materials and Methods from the changes in pH and the corresponding concentrations of open-chain forms with time in unbuffered solutions of the four AHLs (curves similar to those in Fig. 6 for C4-HSL). The quoted figures relate to a standard concentration of AHL (3.7 mM), and the errors in the calculation are estimated to be <20%.