TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype and/or phenotypeaSource or reference
Strains
    OG1RFWild-type E. faecalis strain; Gel+ Spr+ Rifr Fusr18
    TX5128OG1RF gelE transposon mini-γδ insertion mutant; Gel Spr Rifr Fusr Kanr28
    TX5240OG1RF fsrA insertional mutant; Gel Spr Rifr Fusr Kanr23
    TX5242OG1RF fsrC insertional mutant; Gel Spr Rifr Fusr Kanr23
    TX5243OG1RF sprE insertional mutant; Gel+ Spr Rifr Fusr Kanr23
    TX5264OG1RF gelE in-frame deletion mutant (bp 252 to 1490), Gel Spr+ Rifr FusrThis study
    TX5266OG1RF fsrB in-frame deletion mutant (bp 79 to 684), Gel Spr Rifr Fusr29, 22
    TX5266.01TX5266 harboring plasmid pTEX5249; Gel+ Spr+ Rifr Fusr EmrThis study
    TX5266.OSTX5266 harboring plasmid pAT18; Gel Spr Rifr Fusr EmrThis study
    E007E. faecium clinical isolate7
Plasmids
    pAT18E. coli-E. faecalis shuttle vector; Emr33
    pTEX4577E. faecalis suicide vector derived from pBluescript SK(−); Kanr28
    pTEX5249pAT18 containing 6-kb PstI/BglII fragment encoding fsrA, fsrB, and fsrC; Emr23
  • a Abbreviations: Rif, rifampin; Fus, fusidic acid; Kan, kanamycin; Em, erythromycin; Gel, gelatinase; Spr, serine protease; r, resistance.