TABLE 1.

Strains and plasmids used in this study

Plasmid or strainRelevant genotype or characteristic(s)Source or reference
pBluescript II KS(+) E. coli phagemid cloning vectorStratagene
pTS1 S. aureus-E. coli temperature-sensitive shuttle vector 19
pEC4pBluescript II KS(+) with ermB inserted into ClaI site 5
pGAS-4pBluescript II KS(+) containing an internal fragment of the acnA gene of RN6390 inserted into SmaI siteThis study
pGAS-5pGAS-4 containing the ermB cassette of pEC4 inserted into the NsiI site of the acnA fragmentThis study
pGAS-6The acnA::ermB PCR product from pGAS-5 inserted into the SmaI site of pTS1This study
RN4220Restriction-negative S. aureus 32
SA564Recent human S. aureus isolateJ. M. Musser
SA564-acnA::ermBSA564 containing an ermB insertion into acnAThis study