TABLE 2.

Cell migration to the lung after challenge of memory mice with aerosol M. tuberculosisa

Days postchallenge% CD4 + T cells (SEM)% CD8 + T cells (SEM)% Macrophages (SEM)No. of CFU/lung (105) (SEM)
CCR2−/−CCR2+/+CCR2−/−CCR2+/+CCR2−/−CCR2+/+CCR2−/−CCR2+/+
1820.2 (2.6)27.6 (7.5)18.4 (2.4)16.0 (2.5)NDND3.1 (0.25)5.0 (0.4)
2821.9 (1.5)22.5 (1.3)21.1 (0.9)17.3 (1.2)11.2 (0.8)∗∗18.3 (1.14)5.8 (1.56)5.2 (1.66)
4916.4 (1.7)19.0 (1.4)18.4 (1.1)16.5 (0.9)8.2 (1.98)∗16.5 (1.62)6.4 (1.27)6.8 (1.94)
  • a C57BL/6 mice and CCR2−/− mice were infected with M. tuberculosis H37Rv (50 CFU) via the aerosol route. At 4 months postinfection, the mice were drug treated for 2 months. The mice were then challenged with aerosol M. tuberculosis H37Rv (50 CFU), and flow cytometric analysis was performed at the times indicated in the table. ∗, P = 0.02; ∗∗, P = 0.001.