TABLE 6.

Effect of preopsonization on the induction of TNF-α and IL-12 production by macrophages

Experimental groupPresence of opsonizationaProduction of cytokine (pg/ml)b
TNF-αIL-12
Laboratory conditions430 ± 4065 ± 10
+505 ± 5269 ± 4
Anaerobiosis110 ± 14c10 ± 2c
+293 ± 26c18 ± 3c
pH 6.0135 ± 37c8 ± 1c
+232 ± 20c17 ± 5c
  • a M. tuberculosis was opsonized with 10% serum at 37°C for 30 min and then used to infect macrophages in the presence of 10% heat-inactivated serum.

  • b Cytokine production was measured by ELISA. Results are means × standard deviations. The experiment was repeated three times. Results are means ± standard deviations.

  • c P < 0.05 compared with laboratory conditions.