TABLE 5.

Effect of protein synthesis inhibition on M. tuberculosis invasion of macrophages

M. tuberculosis strainConditionaUptake (% of inoculum) of M. tuberculosis in the presence or absence of antibioticb
AbsencePresence
H37RvLaboratory18 ± 417 ± 3
pH 6.056 ± 921 ± 4c
Anaerobiosis57 ± 1123 ± 5c
ErdmanLaboratory23 ± 722 ± 2
pH 6.061 ± 627 ± 5c
Anaerobiosis58 ± 726 ± 4c
  • a Bacteria were exposed to the conditions for 4 h and then incubated with macrophages in the presence of 10% heat-inactivated serum. Results are means ± standard deviations.

  • b Bacteria were treated with rifampin (0.5 μg/ml) as described in Materials and Methods.

  • c P < 0.05 compared with invasion without prior incubation with antibiotic.