TABLE 3.

Phenotypic characteristics of mutant strains used in this studya

StrainGrowth rate (% of R6)Maximum OD600bLog no. of coloniesRelative in vivo virulence
R61000.752.901.0
vicKKO300.460.481.4
vicKst500.660.601.8
vicKKOP900.532.070.9
vicXKO600.571.880.8
SOBvicR900.653.601.8
SOBvicK900.602.712.5
SOBvicX900.592.831.0
  • a This table indicates the phenotypic characteristics of the different mutant strains, as far as growth rate, competence, and in vivo virulence characteristics of the SOBvicK mutant are concerned. The growth rate was determined with respect to S. pneumoniae R6. Transformability is shown as the logarithmic number of transformants obtained. Experiments were repeated three times in triplicate, and the number of transformants was averaged. The in vivo virulence data are the calculated mean survival times of the mutant strains relative to that for a wild-type infection. Results of three in vivo virulence experiments were averaged.

  • b OD600, optical density at 600 nm.