TABLE 1.

Plasmids and bacterial strains used in this study

Plasmid, protein, or strainDescriptionSource or reference
Plasmids and proteins
    EGFP-RhoG(wt)Wild-type RhoG with N-terminal EGFP tagThis study
    EGFP-RhoG(G12V)GTPase-deficient (constitutively active) RhoG with N-terminal EGFP tagThis study
    EGFP-RhoG(T17N)Dominant-negative RhoG with N-terminal EGFP tagThis study
    EGFP-Rac1(wt)Wild-type Rac1 with N-terminal EGFP tagThis study
    pRNAT-Scrambled KDaRNAi plasmid encoding a control (nontargeting) shRNAThis study
    pRNAT-RhoG KDRNAi plasmid encoding a RhoG-targeting shRNAThis study
    pRNAT-Rac1 KDRNAi plasmid encoding a Rac1-targeting shRNAThis study
    mCFP-RhoG(wt)bWild-type RhoG with N-terminal mCFP tagThis study
    mCFP-RhoG(G12V)GTPase-deficient RhoG with N-terminal mCFP tagThis study
    mCFP-RhoG(T17N)Dominant-negative RhoG with N-terminal mCFP tagThis study
    mCFP-RhoG(F37A)Effector binding mutant RhoG with N-terminal mCFP tagThis study
    mCFP-RhoG(Y40C)Effector binding mutant RhoG with N-terminal mCFP tagThis study
    ELMO-mYFPRat ELMO2(AA1-362) with C-terminal mYFP tagThis study
    mCFP-Rac1(wt)Wild-type Rac1 with N-terminal mCFP tag57
    PBD-mYFPbp21 binding domain from Pak1 with C-terminal mYFP tag57
    myc-mCFP-RhoG(G12V)GTPase-deficient RhoG with N-terminal myc-mCFP tagThis study
    myc-mCFP-Rac1(G12V)GTPase-deficient Rac1 with N-terminal myc-mCFP tagThis study
    Arf6-HAWild-type Arf6 with C-terminal HA tag15
Strains
    YPIII(P+)Parental Y. pseudotuberculosis strain (naturally yopT and sycT deficient)4
    YPIII(P)Plasmid-cured Y. pseudotuberculosis strain4
    YPIII yopBYPIII(P+) with in-frame yopB deletion30
    YP17YPIII(P+) with in-frame yopE and yopH deletions3
    YP17/pYopEYP17 with plasmid carrying yopE under the control of an inducible promoter3
    YP17/pYopTYP17 with plasmid carrying yopT and sycT under the control of the yopH promoter52
  • a KD, knockdown.

  • b mCFP and mYFP, monomeric cyan and yellow fluorescent proteins, respectively.