TABLE 1.

Bacterial strains and plasmids

Strain or plasmidaDescriptionbSource or reference
Strains
    E. coli DH5αφ80dlacZΔM15 ΔlacU169 deoR recA endA1Life Technologies
    H. influenzae DB117Laboratory strain, rec-1, capsule-deficient serotype d, with nonsense mutation in hap23a
Plasmids
    pGJB103E. coli-H. influenzae shuttle vector; Tcr27a
    pJS106pGJB103 with a 6.7-kb PstI fragment containing hap26, 27
    pMLD100pUC19 with a 6.7-kb PstI fragment containing hap13
    pHapS243ApJS106 with S243A mutation13
    pHapL1036SpJS106 with L1036S mutation5
    pHapL1036FpJS106 with L1036F mutationThis study
    pHapL1035SpJS106 with L1035S mutationThis study
    pHapL1035TpJS106 with L1035T mutationThis study
    pHapL1035EpJS106 with L1035E mutationThis study
    pHapE1045LpJS106 with E1045L mutationThis study
    pHapE1045ApJS106 with E1045A mutationThis study
    pHapF1077LpJS106 with F1077L mutationThis study
    pHapF1077L/L1036SpJS106 with F1077L/L1036S mutationsThis study
    pHapF1077L/L1036FpJS106 with F1077L/L1036F mutationsThis study
    pHapF1067LpJS106 with F1067L mutationThis study
    pHapV1066LpJS106 with V1066L mutationThis study
    pHapQ1033ApJS106 with Q1033A mutationThis study
    pHapQ1033EpJS106 with Q1033E mutationThis study
    pHapS1034FpJS106 with S1034F mutationThis study
    pHapS1034EpJS106 with S1034E mutationThis study
    pHapN1037RpJS106 with N1037R mutationThis study
    pHapA1038SpJS106 with A1038S mutationThis study
    pHapL1039SpJS106 with L1039S mutationThis study
    pHapE1040SpJS106 with E1040S mutationThis study
    pHapY137ApJS106 with Y137A mutationThis study
    pHapK240ApJS106 with K240A mutationThis study
    pHapL263RpJS106 with L263R mutationThis study
    pHapN274RpJS106 with N274R mutationThis study
    pHapR264ApJS106 with R264A mutationThis study
    pHapE265ApJS106 with E265A mutationThis study
    pHapE265RpJS106 with E265R mutationThis study
    pHapE265WpJS106 with E265W mutationThis study
  • a Plasmids encoding wild-type hap or mutant hap derivatives were expressed in H. influenzae strain DB117.

  • b Tcr, tetracycline resistance.