TABLE 3.

Autoproteolysis phenotypes of Hap mutant derivatives

Wild type or mutationaCleavage site or subsitebDescriptionc
Hap wild typeNA1°, 2°, and 3° cleavage
HapL1036SP1 (1°)No 1° cleavage; 2°, 3°, and 4° cleavage
HapL1036FP1 (1°)1° cleavage slightly diminished, enhanced secondary cleavage
HapF1077LP1 (3°)Slightly enhanced 3° cleavage
HapF1077L/L1036SP1 (3°) + P1 (1°)Enhanced 3° cleavage
HapF1077L/L1036FP1 (3°) + P1 (1°)Enhanced 3° cleavage
Hap F1067LP1 (4°)Enhanced 4° cleavage, some 1° and 3° cleavage
HapL1035SP2 (1°)Very little 1° cleavage, enhanced 2° cleavage
HapL1035TP2 (1°)Minimal autoproteolysis, accumulation of Hap precursor
HapL1035EP2 (1°)No effect on autoproteolysis
HapE1045LP2 (2°)Enhanced 2° cleavage
HapE1045AP2 (2°)Enhanced 2° cleavage
HapV1066LP2 (3°)Enhanced 4°, some 1° and 3° cleavage
HapS1034FP3 (1°)No effect on autoproteolysis
HapS1034EP3 (1°)No effect on autoproteolysis
HapQ1033AP4 (1°)No effect on autoproteolysis
HapQ1033EP4 (1°)No effect on autoproteolysis
HapN1037RP1′ (1°)No effect on autoproteolysis
HapA1038SP2′ (1°)No effect on autoproteolysis
HapL1039SP3′ (1°)No effect on autoproteolysis
HapE1040SP4′ (1°)No effect on autoproteolysis
HapK240AS1No effect on autoproteolysis
HapL263RS1Diminished autoproteolysis, accumulation of Hap precursor
HapN274RS1Diminished autoproteolysis, accumulation of Hap precursor
HapR264AS2Diminished autoproteolysis, accumulation of Hap precursor
HapY137AS2No effect on autoproteolysis
HapE265AS4Diminished autoproteolysis, accumulation of Hap precursor
HapE265WS4Diminished autoproteolysis, accumulation of Hap precursor
  • a All Hap derivatives were expressed in H. influenzae strain DB117.

  • b Numbers in parentheses indicate the primary (1°), secondary (2°), tertiary (3°), or quaternary (4°) cleavage sites. NA, not applicable.

  • c Descriptions are based on visual inspection of the blots in triplicate.