TABLE 2.

Affinity constants of different His-tagged SEN proteins for Lys- and Glu-plasminogena

His-tagged sen proteinLys-plasminogenGlu-plasminogen
Kd (nM)Bmax (102 fmol)Kd (nM)Bmax (102 fmol)
SEN-Wtb12710.702674.79
SEN-ΔK43526011.06002.45
SEN-ΔK434-4353275.99950.7
SEN-K435L1,1191.31,0290.7
SEN-K434-435LND0.6ND0.13
SEN-K428-434-435LND0.5ND0.08
  • a Kd, equilibrium dissociation constant; Bmax, maximal amount of radioligand bound per well containing the specific SEN recombinant protein; ND, undeterminable.

  • b SEN-Wt, wild-type SEN.