TABLE 2.

Plasmids used in this study

PlasmidRelevant characteristicsaReference or source
Intimin plasmids
    pMW103Truncated eae (bp 1959-2804) in pQE31 (Qiagen)13
    pNR37 eae from pMW103, plant optimizedThis study
Plant expression vectors
    pIBT210CaMV 35S promoter, TEV 5′ UTR, Vsp 3′ UTR18
    pBTI210.3CaMV 35S promoter, TMVΩ 5′ UTR, Vsp 3′ UTRHugh Mason, while at Boyce Thompson Institute for Plant Research
    pBTI210.4CaMV 35S promoter, TEV 5′ UTR, VspB 3′ UTR, with plant-specific signal peptide sequenceHugh Mason, while at Boyce Thompson Institute for Plant Research
    pGPTV-KanBinary vector, Kanr4
Plant-specific intimin expression vectors
    pNR49 eae from pNR37 with signal peptide sequence from pBTI210.4This study
    pNR50 eae from pNR37This study