TABLE 3.

Oligonucleotide sets for eae (nucleotides 1959 to 2804) plant expression optimization by QuikChange site-directed mutagenesis

OligonucleotideSequencea
1-forwardGGGTCAGCCAGTTAACAACCAATCCGTTACATTCTCAACAAACTTTGG
1-reverseCCAAAGTTTGTTGAGAATGTAACGGATTGGTTGTTAACTGGCTGACCC
2-forwardGGCTGCAATATGGTCAATTTAAACTGAAAGCAAGCGG
2-reverseCCGCTTGCTTTCAGTTTAAATTGACCATATTGCAGCC
3-forwardGGGAAAGTCACTTTGAATGGTAAAGGCTCTGTCGTAATTAAAGCC
3-reverseGGCTTTAATTACGACAGAGCCTTTACCATTCAAAGTGACTTTCCC
4-forwardGGTGATAAGCAAACAGTAAGTTACACTATCAAGGCACCGTCG
4-reverseCGACGGTGCCTTGATAGTGTAACTTACTGTTTGCTTATCACC
5-forwardGCTATGTCCATTTGCAAAAACTTGTTACCATCCACACAGACGG
5-reverseCCGTCTGTGTGGATGGTAACAAGTTTTTGCAAATGGACATAGC
6-forwardGGTATTGTCAGATATCTATGACTCATGGGGGGCTGCAAACAAGTATAGCC
6-reverseGGCTATACTTGTTTGCAGCCCCCCATGAGTCATAGATATCTGACAATACC
7-forwardGGATTAAACAGACATCTAGTGAGCAACGTTCTGGAGTATCAAGC
7-reverseGCTTGATACTCCAGAACGTTGCTCACTAGATGTCTGTTTAATCC
  • a Sites of mutagenesis are indicated in bold.