TABLE 2.

P. multocida genes up-regulated during growth within all three infected chickens

GenePrecise or predicted function (COGs categorya)Expression valueb
c1c2c3
asnAAmmonia-dependent asparagine synthetase (E)3.22 ± 0.771.24 ± 0.32.71 ± 0.37
aspCAspartate transaminase (E)1.09 ± 0.371.17 ± 0.31.51 ± 0.32
dcaAUnknown (R)1.27 ± 1.342.18 ± 0.432.86 ± 0.9
dppADipeptide transport system permease (E)0.88 ± 0.571.95 ± 0.453.5 ± 0.33
gdhAGlutamate dehydrogenase (E)3.01 ± 0.271.51 ± 0.393.27 ± 0.91
gltACitrate synthase (C)1.04 ± 0.391.35 ± 0.432.05 ± 0.45
ilvHAcetolactate synthase (E)1.21 ± 0.991.82 ± 0.411.33 ± 0.23
napAPeriplasmic nitrate reductase (C)2.33 ± 0.262.13 ± 0.511.69 ± 0.14
napCPeriplasmic nitrate reductase (C)1.97 ± 0.532.28 ± 0.811.08 ± 0.85
napFPeriplasmic nitrate reductase (C)3.7 ± 0.172.58 ± 0.172.29 ± 0.43
PM0092Sigma-54 modulation protein (J)1.4 ± 0.410.83 ± 0.11.63 ± 0.14
PM0287Oxidoreductase (R)0.99 ± 0.323.07 ± 0.172.1 ± 0.3
PM0601Carbohydrate transport and metabolism (G)0.9 ± 1.180.76 ± 0.721.36 ± 1.05
PM1460Phosphoglycerate transport system activator (T)1.26 ± 1.151.23 ± 0.41.29 ± 0.61
ppcPEP carboxylase (C)3.07 ± 0.591.39 ± 0.493.04 ± 0.61
ptfAType IV fimbrial subunit (N)0.89 ± 0.790.83 ± 0.450.9 ± 0.44
purHPurine biosynthesis (F)1.26 ± 0.120.87 ± 0.161.21 ± 0.25
  • a COGs functional categories (44). The full functional categories associated with each COGs letter designation are given in the legend to Fig. 3. Where no precise function is apparent from amino acid homology, the general COGs category title is listed under the predicted function.

  • b Expression values are expressed as the log2 of (average experimental intensity/average control intensity) ± 1 standard deviation. Therefore, identical experimental and control expression values would be 0.0.