Immunogen and rabbit | ELISA titer to | FVO/3D7 titer ratio | |
---|---|---|---|

PpAMA1 FVO | PpAMA1 3D7 | ||

PpAMA1 FVO | |||

STO1 | 107,931 | 42,643 | 2.53 |

STO2 | 64,139 | 27,739 | 2.31 |

STO3 | 87,124 | 35,602 | 2.45 |

STO4 | 167,085 | 59,528 | 2.81 |

Geometric mean | 100,192.7 | 39,790.9 | 2.52 |

PpAMA1 3D7 | |||

STO5 | 38,271 | 98,540 | 0.39 |

STO6 | 37,534 | 94,441 | 0.40 |

STO7 | 45,403 | 122,013 | 0.37 |

STO8 | 29,815 | 65,086 | 0.46 |

Geometric mean | 37,342.5 | 92,718.6 | 0.40 |

PpAMA1 FVO plus PpAMA1 3D7 | |||

STO17 | 73,346 | 78,890 | 0.93 |

STO18 | 62,356 | 58,240 | 1.07 |

STO19 | 248,275 | 264,520 | 0.94 |

STO20 | 65,517 | 80,499 | 0.81 |

Geometric mean | 92,872.1 | 99,454.2 | 0.93 |

↵

*a*Rabbits were immunized twice, 4 weeks apart (days 0 and 28), each time intramuscularly with 25 μg of antigen emulsified in Montanide ISA720 (either 25 μg of PpAMA1 FVO, 25 μg of PpAMA1 3D7, or 12.5 μg of PpAMA1 FVO plus 12.5 μg of PpAMA1 3D7). Titers shown are from bleeds 2 weeks after the second immunization (day 42).