TABLE 4.

Antibody titers following immunization of rabbits with PpAMA1 FVO, PpAMA1 3D7, or a combination of the two antigensa

Immunogen and rabbitELISA titer toFVO/3D7 titer ratio
PpAMA1 FVOPpAMA1 3D7
PpAMA1 FVO
    STO1107,93142,6432.53
    STO264,13927,7392.31
    STO387,12435,6022.45
    STO4167,08559,5282.81
        Geometric mean100,192.739,790.92.52
PpAMA1 3D7
    STO538,27198,5400.39
    STO637,53494,4410.40
    STO745,403122,0130.37
    STO829,81565,0860.46
        Geometric mean37,342.592,718.60.40
PpAMA1 FVO plus     PpAMA1 3D7
    STO1773,34678,8900.93
    STO1862,35658,2401.07
    STO19248,275264,5200.94
    STO2065,51780,4990.81
        Geometric mean92,872.199,454.20.93
  • a Rabbits were immunized twice, 4 weeks apart (days 0 and 28), each time intramuscularly with 25 μg of antigen emulsified in Montanide ISA720 (either 25 μg of PpAMA1 FVO, 25 μg of PpAMA1 3D7, or 12.5 μg of PpAMA1 FVO plus 12.5 μg of PpAMA1 3D7). Titers shown are from bleeds 2 weeks after the second immunization (day 42).