TABLE 2.

Oligonucleotide primers used in this studya

NameSequence (5′-3′)
1721RbTTAAAGACATGTCCGTCAATCC
SpeL_FbATGAGAATTTTTTTACACCAG
SpeL_FcutbACCATGGGAAGAGACTATTAATATTAAGG
SpeL_RbCGAATTCATTTTCTTTGTTTGTGAAT
117bAAATGGCGTCATTTTCTCA
SpeM_FbATGTTAGGAGAAAAAATG
SpeM_FcutbACCATGGTTTTCAGATGCTGTGTTGG
SpeM_RbCGGATCCATTTTTAGAAAAATCTTCG
SpeL_8169bACATATTAACCAAACGACAATTC
SpeM_8952bAGTTATATCTAGCAATGTTTCTCCA
Param147bCCAGCGCAAGAAAGACG
Param112bACATATTAACCAAACGACAATTC
Param134bAGTTATATCTAGCAATGTTTCTCCA
1220LbCAGCACCTTCCTCTTTCTCG
1220RbGGAAAAAGAGGGACGCAAG
G1295LbGGATGAGTGAATAAATCGGTAAAC
G5-358D2bCTTTTGACTGATAGTGACAAGCTG
SBBP4004.2bCTTGTGTGTGTATCGCTTGCTC
1237_RevbTCATGCGGCAACACATA
SpeL_5TQb,cTCGATGTTTTTGGTCTTGCATT
SpeL_3TQb,cCCGGCTCCTTTTCTCTCATTAA
SpeL_probec6FAM-AAAGATCAAATACATTCAATCAATGGTGGTCTCGTTAAA-TAMRA
SpeM_5TQb,cAAAGATGTAATTAATAGAACCAATATGAAGATAACA
SpeM_3TQb,cAATTATCATCGTCCCAGACTCTAGTTTT
SpeM_probec6FAM-AGAAAATTGGCACCCAGTTAATATTTAACACGAATGA-TAMRA
SpeB_5TQcCGCACTAAACCCTTCAGCTCTT
SpeB_3TQcACAGCACTTTGGTAACCGTTGA
SpeB_probec6FAM-GCCTGCGCCGCCACCAGTA-TAMRA
GyrA_5TQcCGACTTGTCTGAACGCCAAA
GyrA_3TQcTTATCACGTTCCAAACCAGTCAA
GyrA_probec6FAM-CGACGCAAACGCATATCCAAAATAGCTTG-TAMRA
  • a Primers were designed based on the genome sequence of GAS strain MGAS8232 (serotype M18) (33).

  • b Primers used for sequencing the speL-speM locus.

  • c Primers and probes used for real time RT-PCR analysis (TaqMan assays). Probes were labeled at the 5′ end with the reporter dye 6-carboxyfluorescein (6FAM) and at the 3′ end with the quencher 6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA).