TABLE 3.

P. multocida genes down-regulated during growth within all three infected chickens

GenePrecise or predicted function (COGs categorya)Expression valueb
c1c2c3
glpBGlycerol-3-phosphate dehydrogenase (E)−1.05 ± 0.34−1.53 ± 0.21−1.23 ± 0.21
glpCGlycerol-3-phosphate dehydrogenase (C)−0.94 ± 0.2−1.35 ± 0.18−0.91 ± 0.15
hbpAHeme-binding protein A precursor (E)−3.77 ± 0.37−3.02 ± 0.17−2.18 ± 0.44
murZUDP-GlcNAc enolpyruvyl transferase (F)−0.95 ± 0.31−1.06 ± 0.3−1.78 ± 0.23
PM0038Amidotransferase (R)−2.94 ± 0.16−2.63 ± 0.32−2.51 ± 0.69
PM0159Unknown (R)−2.05 ± 0.23−2.55 ± 0.37−2.71 ± 0.41
PM0243Nucleotide transport and metabolism (F)−1.24 ± 0.14−1.07 ± 0.2−1.2 ± 0.14
PM0310Unknown (−)−2.2 ± 0.66−1.02 ± 2.3−1.81 ± 0.17
PM0335Glutamate symport protein (R)−2.15 ± 0.47−2.79 ± 1.12−4.44 ± 0.44
PM0402Unknown (R)−1.43 ± 0.31−1.44 ± 0.29−1.46 ± 0.24
PM0591Unknown (S)−0.94 ± 0.68−1.08 ± 0.42−1.49 ± 1.59
PM0682Unknown (−)−1.23 ± 0.82−1.65 ± 0.91−1.7 ± 0.5
PM0761Unknown (S)−1 ± 0.23−1.15 ± 0.11−2.02 ± 0.26
PM0965Unknown (−)−2.62 ± 0.85−2.77 ± 0.45−1.45c
PM1233Amidotransferase (H)−1.87 ± 1.06−2.01 ± 0.63−0.92 ± 0.12
PM1566Unknown (S)−1.84 ± 0.41−1.66 ± 0.21−1.84 ± 0.21
PM1889Unknown (S)−1.22 ± 0.47−1.22 ± 0.2−1.96 ± 0.48
PM1926Nucleotide transport and metabolism (F)−0.9 ± 0.37−1.19 ± 0.16−1.69 ± 0.16
PM1932Unknown (−)−0.96 ± 0.38−1.27 ± 0.65−1.58 ± 0.88
ptsBSucrose-specific PTSII (G)−1.76 ± 0.52−2.09 ± 0.69−1.84 ± 0.38
rpL34Ribosomal protein L34 (J)−1.07c−1.67c−1.15 ± 1.95
rpL35Ribosomal protein L35 (J)−1.95 ± 0.21−2.07 ± 0.33−1.78 ± 0.35
rseBSigma-E regulatory protein (T)−2.08 ± 0.18−1.5 ± 0.16−1.21 ± 0.14
  • a COGs functional categories (44). The full functional categories associated with each COGs letter designation are given in the legend to Fig. 3. Where no precise function is apparent from amino acid homology, the general COGs category title is listed under the predicted function.

  • b Expression values are expressed as the log2 of (average experimental intensity/average control intensity) ± 1 standard deviation. Therefore, identical experimental and control expression values would be 0.0.

  • c No standard deviation could be calculated as the data were derived from fewer than three values.