TABLE 3.

Pyrogenicity and lethality of rSpeL and rSpeM

ToxinPyrogenicitya (avg temp increase in °C)Models of lethality (no. dead/no. total animals)
Endotoxin enhancementbMiniosmotic pumpc
SpeL0.873/32/3
SpeM0.673/30/3
SpeC1.503/33/3
PBS−0.070/3NDd
  • a Three rabbits were used for each assay. Animals were injected intravenously with 5 μg of recombinant protein per kg at 0 h, and temperatures were recorded at 4 h. A change of 0.5°C or greater is considered to be significant in this model (29, 31).

  • b Mortality was recorded for a 48-h period after injection of endotoxin.

  • c Mortality was recorded for 15 days after subcutaneous implantation of a miniosmotic pump.

  • d ND, not determined.