TABLE 1.

Primers and amplicon sizes for loci included in the PCR assay

LocusPrimer designationNucleotide sequence of primerAmplicon size(s) (bp)
bfpAbfpA-F115′-GTCTGCGTCTGATTCCAATA-3′408, 414
bfpA-R15′-TCAGCAGGAGTAATAGC-3′
toxBtoxB.911F5′-ATACCTACCTGCTCTGGATTGA-3′602
toxB.1468R5′-TTCTTACCTGATCTGATGCAGC-3′
stx11A.251F5′-GGGATAGATCCAGAGGAAGG-3′624
1A.832R5′-CCGGACACATAGAAGGAAACTC-3′
stx22A.506F5′-CTGGCGTTAATGGAGTTCAG-3′383
2A.848R5′-CCTGTCGCCAGTTATCTGAC-3′
eaeeae-F15′-ACTCCGATTCCTCTGGTGAC-3′389
eae-R35′-TCTTGTGCGCTTTGGCTT-3′
terCterC.106F5′-TATGCACCGTGATGACAAGC-3′210
terC.275R5′-GGCGAACCAGGAGAAGATTG-3′
ureAureA.109F5′-TAACTATCCCGAATCCGTGG-3′146
ureA.213R5′-GGGATCATTTCTGGTATGCCT-3′
ihaiha.138F5′-ACGCAGCCGCCAGTGTT-3′186
iha.282R5′-CCATCAATCAGTATCAGCGTGTAA-3′
invGinvG.481F5′-TGACCTGGTCGTTAATGCTG-3′133
invG.572R5′-CGCCACGTAACTCATAAGTCC-3′
spaPspaP.143F5′-GGACTTCAGCAAGTGCCATC-3′173
spaP.275R5′-ACCACTCATGCCTGTCTCAA-3′
Z4326ShET.1137F5′-TCATGCAGACGCAATAAAGG-3′125
ShET.1221R5′-GATACGCAAGAAGTAATCCTGG-3′
fyuAfyuA.924F5′-GCAGCAGCAGCATTATTCG-3′495
mdhmdh.269F5′-GGTATGGATCGTTCCGACCT-3′304
mdh.530R5′-GGCAGAATGGTAACACCAGAGT-3′