Description of the Bdr proteins of B. burgdorferi B31MI and summary of production data

Bdr paralogaMassbPlasmidBdr protein productionc under various conditions
Temp (°C)HASerum deprivation for:
23370 h24 h48 h
BdrD1 (BBL35)22.1cp32-8++++++
BdrD2 (BBM34)25.4cp32-6++++++
BdrD3 (BBO34)22.0cp32-7++++++
BdrD4 (BBP34)24.1cp32-1++++++
BdrD5 (BBQ42)20.6lp56++++++
BdrD6 (BBS37)22.7cp32-3++++++
BdrD10 (BBN34)20.7cp32-9++++++
BdrE1 (BBL27)22.4cp32-8++++↓1.5[0]↓1.5[0]
BdrE2 (BBN27)22.3cp32-9++++
BdrE3 (BBO27)23.7cp32-7+↓1.5(23)↑2.5(37)+↓1.5[0]↓2.0[0]
BdrE4 (BBR27)20.3cp32-4++↑4.4(37)+↓1.5[0]↓1.5[0]
BdrE5 (BBS29)24.1cp32-3+↓4.8(23)↑6.4(37)+↓1.5[0]↓1.5[0]
BdrE6 (BBQ34)27.3lp56+↓1.9(23)↑1.6(37)+↓2.0[0]↓2.0[0]
BdrF1 (BBF03)20.0lp28-1++++
BdrF2 (BBG33)30.6lp28-2++↑3.4(37)+++
BdrF3 (BBH13)25.8lp28-3++++++
  • a The ORF designation assigned by the Institute for Genomic Research is shown in parentheses after the Bdr paralog.

  • b Molecular mass (in kilodaltons).

  • c A plus sign (+) indicates production of the protein, while a minus sign (−) indicates that production was not detected by immunoblotting. For those proteins that are not expressed during cultivation under normal conditions, two plus signs indicate that production increased with time during serum deprivation. The numerical values listed indicate fold changes in production level (↑ indicating fold increase and ↓ indicating fold decrease) relative to the production level at a certain temperature or time (i.e., 0 h, or not serum starved) (indicated in parentheses or brackets, respectively, in superscript). All values, except those for BdrE3 were determined by scanning densitometry. The values for BdrE3 could not be determined by this approach due to the close proximity of other Bdr bands and instead were visually estimated.

  • d HA, host-adapted bacteria.