TABLE 1.

Bacterial strains and plasmids

Strain or plasmidGenotype or descriptionSource or reference
Strains
    S. aureus
        8325-4Wild-type cured of known prophages41
        RN4220Accepts foreign DNA (r m+)30
        COLMethicillin resistant29
        PC6911agrΔ::tetM Tcr in 8325-4 background4
        PC1839sarA::km Kmr in 8325-4 background5
        LH9ebh::pAZ4353 Emr in 8325-4 backgroundThis work
        LH10ebh::pMUTHEX in 8325-4 backgroundThis work
        LH11ebh::pMUTHEX in PC6911 backgroundThis work
        LH12ebh::pMUTHEX in PC1839 backgroundThis work
        LH17ebh::pAZ4353 Emr in COL backgroundThis work
        SRC001ebh::pMUTHEX in COL backgroundThis work
    E. coli
        DH5αφ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17 (rK mK+) supE44 relA1 deoR Δ(lacZYA-argF)U169Promega
        BL21(DE3)F ompT gal [dcm] [lon] hsdSB (rB mB)Novagen
Plasmids
    pAZ106Promoterless, transcriptional lacZ fusion vector; Apr (E. coli), Emr (S. aureus)27
    pAZ4353pAZ106-based vector containing a ∼1.6-kb BamHI/EcoRI-cut fragment of ebh; Apr (E. coli), Emr (S. aureus)This work
    pMUTIN4Promoterless, transcriptional lacZ fusion vector; Apr (E. coli), Emr (S. aureus)52
    pMUTHEXpMUTIN4-based vector containing a ∼1.2-kb BamHI/HindIII-cut fragment of ebh, creating an ebh::lacZ fusion; Apr (E. coli), Emr (S. aureus)This work
    pET24-dHis6 tag overexpression vector; KmrNovagen
    pETH2pET24-d containing ∼1-kb internal ebh fragment encoding H2 polypeptide; KmrThis work