TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Source or reference
Strains
    A. hydrophila
        SSUCDCa
        SSU-RRifr strain of A. hydrophila SSULaboratory stock
        M1gidA transposon mutant of A. hydrophila SSU-R; Rifr Sp/SmrThis study
        M19gidA transposon mutant of A. hydrophila SSU-R; Rifr Sp/SmrThis study
        MgidAgidA isogenic mutant of A. hydrophila SSU-R generated by double crossover; Rifr Kmr, sucrose resistanceThis study
        SSU-R/pBR322SSU-R containing pBR322 vector; Rifr TcrThis study
        M19/pBR322gidA transposon mutant M19 containing pBR322 vector; Rifr Sp/Smr TcrThis study
        MgidA/pBR322gidA isogenic mutant containing pBR322 vector; Rifr Kmr TcrThis study
        M19/pBRgidAgidA transposon mutant M19 complemented with the gidA gene via pBR322 vector; Rifr Sp/Smr TcrThis study
        MgidA/pBRgidAgidA isogenic mutant complemented with the gidA gene via pBR322 vector; Rifr Kmr TcrThis study
        SSU66A. hydrophila SSU-R with chromosomally integrated act::phoA fusion; Rifr Gmr sucrose sensitiveThis study
        M19phoATransposon mutant M19 with chromosomally integrated act::phoA fusion; Rifr Gmr Sp/Smr, sucrose sensitiveThis study
        MgidAphoAgidA isogenic mutant with chromosomally integrated act::phoA fusion; Rifr Gmr Kmr, sucrose sensitiveThis study
        SSU66/pBR322SSU66 containing pBR322 vector; Tcr Rifr Gmr, sucrose sensitiveThis study
        M19phoA/pBR322M19phoA containing pBR322 vector; Tcr Rifr Gmr Sp/Smr, sucrose sensitiveThis study
        MgidAphoA/pBR322MgidAphoA containing pBR322 vector; Tcr Rifr Gmr Kmr, sucrose sensitiveThis study
        M19phoA/pBRgidAM19phoA complemented with the gidA gene via pBR322; Tcr Rifr Gmr Sp/Smr, sucrose sensitiveThis study
        MgidAphoA/pBRgidAMgidAphoA complemented with the gidA gene via pBR322; Tcr Rifr Gmr Kmr, sucrose sensitiveThis study
    E. coli
        DH5αrecA gyrALaboratory stock
        S17-1Streptomycin and trimethoprim resistance, λpirLaboratory stock
Plasmids
    pRK2013Helper plasmid, KmrATCCb
    pBR322Apr TcrAmersham
    pBluescript SKAprStratagene
    pUC-4KContains a 1.2-kb kanamycinr gene cassetteAmersham
    pHP45ΩContains a 2.0-kb Sm/Spr gene cassette45
    pJQ200SKSuicide vector; P15A, sacB Gmr46
    Mini-Tn5 Sm/SpMinitransposon containing Sp/Smr gene cassette delivered by the pUT plasmid19
    pJQ200actphoAVector pJQ200SK containing act::phoA fusion; Gmr53
    pBlueM1pBluescript vector containing a 6.6-kb PstI DNA fragment from M1, which harbored the interrupted gidA gene generated by mini-Tn5 transpositionThis study
    pBlueM19pBluescript vector containing a 6.6-kb PstI DNA fragment from M19, which harbored the interrupted gidA gene generated by mini-Tn5 transpositionThis study
    pBlgidApBluescript vector containing coding region of gidA gene of A. hydrophilaThis study
    pBlgidA-KmgidA gene in plasmid pBluegidA interrupted at the BglII site with a Kmr gene cassetteThis study
    pJQgidA-KmVector pJQ200SK containing a Kmr gene cassette interrupting the gidA gene for generating a gidA isogenic mutant, MgidAThis study
    pBRgidAA. hydrophila gidA gene with its putative promoter region cloned in pBR322 at the EcoRI/PstI sitesThis study
  • a CDC, Centers for Disease Control and Prevention, Atlanta, Ga.

  • b ATCC, American Type Culture Collection.