TABLE 5.

Serovar Typhimurium strain SL1344 mutants lacking SPI-1 effector proteins: invasion of COS7 tissue culture cellsa

StrainRelevant genotypeInvasiveness (%)bNo. of assaysP vs:
TTSSEffectorsM556M557
SL1344Wild typeWild type11724NS<0.001
SB161invGWild type0.0623<0.001NS
M556sseDWild type10060.002
M557sseD invGWild type0.1660.002
M715sseDsipA73.26NS0.002
M704sseDsopB1346NS0.002
M719sseDsopEE235.860.0020.002
M716sseDsopBEE20.4160.002NS
M722sseDsopEE2 sipA32.360.0040.002
M707sseDsopBE sipA14.260.0020.002
M717sseDsopBE2 sipA91.26NS0.002
M708sseDsopBEE2 sipA0.2860.002NS
  • a The data were analyzed by using the Mann-Whitney U test (see Materials and Methods). NS, not significant (P ≥ 0.05).

  • b The invasiveness of M556 was defined as 100% (see Materials and Methods).