TABLE 3.

Genes showing greatest down-regulation in the M. tuberculosisΔ sigF mutant strain at different growth phasesa

PhaseMT no.Rv no.DescriptionPhaseMT no.Rv no.Description
Exponential
25032428Alkyl hydroperoxide reductase C
16281592cCHP
23042244Acyl carrier protein
23032243Malonyl CoA-acyl carrier protein
03110297PE-PGRS 5
10411013Polyketide synthase pKS16
33853286cSigF
29122846cEfflux protein
10200991cHP
35273418cChaperonin, 10 kDa
02650251cHSP20 family
05440522Amino acid permease
22782220Glutamine synthetase
27092633cHP
37763675HP
16591623cCytochrome d ubiquinol oxidase
19651914cHP
08820859Thiolase
03720356cCHP
08770854CHP
40333914Thioredoxin
19321884cCHP
18051758Serine esterase, cutinase family
25302455cFerrodoxin oxidoreductase
230522453-oxoacyl-(acyl carrier protein) synthase
02980285PE 5
07280701Ribosomal protein L3
18941846cCHP
2466HP
18541804cHP
37173615cCHP
19461895Zinc-binding dehydrogenase
18171767CHP
34623354HP
12781240Malate dehydrogenase
24452376cCHP
09280905Enoyl-CoA hydratase/isomerase
03270313HP
Early stationary30973017cEsat-6-like protein esxQ
00150012CHP (membrane)
04190406cMetallo-beta-lactamase
04430428cHP
00160013Glutamine amidotransferase
04120402cMembrane protein, MmpL
05870561cMono-oxygenase
05130493cHP
36643559cOxidoreductase
05060487HP
04030392cNADH dehydrogenase
04820466HP
07900766cP450 heme-thiolate protein
08610839Methyltransferase; putative
05960570Ribonucleoside-diphosphate reductase
00110009Peptidyl-prolyl cis-trans isomerase
19251876Bacterioferritin
00980089Methyltransferase, putative
03950383cOrotate phosphoribosyltransferase
03980385Oxidoreductase, putative
00170014cSerine/threonine protein kinase
2369HP
07890765cOxidoreductase
05630538CHP
0576HP
00850078AHP
20982038cSugar ABC tansporter, ATP binding protein
02670254cCobalamine biosynthesis protein cobU
00890082NADH ubiquinone oxidoreductase
00770071CHP, possible maturase
1190HP
05390518HP
04330419HP
05140494Transcriptional regulator, GntR family
05370516cCHP
04630447cCyclopropropane fatty acyl-phospholipid synthase
04810465cDNA-binding protein, putative
05830557Glycosyl transferase
Late stationary23662308HP
07890765cOxidoreductase
28022731HP
3757HP
00820076cHP
2370HP
13941351HP
27022627cHP
19001852Urease accessory protein UreG
09890961HP
04290416CHP
04430428cHP
21172056cribosomal protein S14
20722016HP
07950771HP
00390034HP
20281976cCHP
06120584CHP
04170404Acyl-CoA synthase
32523163cCHP
04890472ctranscription regulator, TetR family
04110401CHP
04320418Hydrolase
28792812 IS1604, transposase
03200307cHP
03910377Transcriptional regulator, LysR family
05870561cMono-oxygenase, FAD-binding, putative
05390518HP
04120402cMembrane protein, MmpL
06900661cHP
06130585cCHP
29302862cCHP, frameshift
04190406cMetallo-beta-lactamase superfamily
30963016HP
02270217cEsterase/lipase, putative
079407703-Hydroxyisobutyrate dehydrogenase
01400132cGlucose-6-phosphate dehydrogenase
  • a Numbers refer to M. tuberculosis CDC1551 MT or H37Rv Rv number. Genes are listed in descending order by magnitude of down-regulation in the absence of SigF. For complete list of down-regulated genes, see the GEO database (www.ncbi.nlm.nih.gov/geo >). Abbreviations: CHP, conserved hypothetical protein; HP, hypothetical protein.