TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidFeature(s)Reference or source
Strains
    B. cenocepacia J2315CF strain; type strain for species and genome sequence strain25, 45
    B. cenocepacia K56-2CF strain and experimental strain used for construction of site-directed gene mutants and infection studies17, 37
    B. cenocepacia CEP54BCF strain representative of Cleveland epidemic strain type 1725
    B. cenocepacia CEP54ADerivative of CF strain CEP54B which lacks BCESM regionThis study
    BCC strainsSelected from published strain panels and Cardiff University collection to be representative of all current genomovars7, 22, 25, 40
    E. coli JM109Standard cloning strain36
    K56-2cciIAHL synthase knockout mutant disrupted at single BsmI site (position 235 in 654-bp CDS)This study
    K56-2amiIAmidase knockout mutant disrupted at single ClaI site (position 233 in 1,401-bp CDS)This study
    K56-2opcIPorin knockout mutant disrupted at single BamHI site (position 734 in 1,001-bp CDS)This study
Plasmids
    pGEM-TPCR product cloning vectorPromega Corporation Inc.
    pUC-TP1.1-kb trimethoprim resistance cassette37
    pJQ200SKGentamicin-resistant, sacB, Mob+, P15A replicon32
    pEGM105-TcpJQ2000SK with tetracycline resistance cassette inserted into BglI site within Gmr gene; suicide vector used for allelic exchange mutagenesisKindly provided by G. Mendrano
    pCciI-TppEGM105-Tc carrying AHL synthase gene interrupted at BsmI site with Tp cassetteThis study
    pAmiI-TppEGM105-Tc carrying amidase gene interupted at ClaI site with Tp cassetteThis study
    pOpcI-TppEGM105-Tc carrying porin gene interupted at BamHI with Tp cassetteThis study