TABLE 2.

Effect of OspB antibodies on attachment of B. burgdorferi to the tick guta

Time (h)No. of spirochetes attached to the tick gut per field with indicated antibody
NRSNRS-Fab2Mab-B22MabB27OspB-Fab2
24121 ± 28159 ± 57265 ± 88221 ± 1427 ± 1.6
48199 ± 45102 ± 51135 ± 3747 ± 1110 ± 3
72227 ± 34117 ± 29297 ± 74205 ± 555 ± 2.3
  • a NCr SCID mice were infected with B. burgdorferi before the administration of antibodies 24 h before tick placement. Engorged ticks were examined from 24 to 72 h after detachment by confocal microscopy to enumerate B. burgdorferi cells in direct contact with tick gut tissue. In each experiment, the entire gut diverticulum was examined, and counts were made from at least three fields from two separate gut diverticula. The average number of microscopic fields per diverticulum was 6; the range was 2 to 7 fields/diverticulum. Data shown are the numbers of spirochetes per field from three independent experiments (means ± standard errors). Differences between the OspB-F(ab2)-treated group and all of the other individual groups at each time period (24, 48, and 72 h) were significant (P values were <0.05). Statistical differences were determined by analysis of variance and Fisher's protected least significant difference test.