TABLE 2.

Gene probes used to map the cci by Southern hybridization

Gene probe namePutative gene productPCR primer sequence (5′ to 3′)PCR annealing temp (°C)Product size (bp)
ForwardReverse
BCAM0236Arsenic resistance proteinGGT TCC TGC TGC TGT ACGTCC TTG CGC TCG CTA TAC54574
cciIAHL synthaseTTG ATC GCG GTC GTA CGAACC GCA ACG CTG GTA AAG50319
BCAM0248Putative IS transposaseGCC GGA AAT CGG AGA AACCGT GAT GCT ACC CAT CCA53512
BCESMOriginal RAPD marker spanning the esmR geneCCA CGG ACG TGA CTA ACACGT CCA TCC GAA CAC GAT621,418
amiIPutative amidaseGCT TTC TTG CGC GCT ACAATG CAC CAC GAT CTT TGC521,821
opcIProbable porin proteinGCC TTT GTC GGA CTG AGCGTC TGT GCG TTT CGA GAG54612
BCAM0278Putative stress proteinGAT CGA CGT CAC CGA AAAGAT CGC GAG CTT CTT CTG52289
BCAM0280Hypothetical proteinCGT CGA GGA TGA ACT GGACCT TAC CGG ACA ACG TGA52317