TABLE 1.

Invasion of KB and HUVEC cells and attachment to HUVEC cells by different fusobacteria

StrainInvasiona (%)Attachment to HUVEC cells (%)
KB cellsHUVEC cells
F. nucleatum 122301.5 ± 0.00.5 ± 0.11.5 ± 0.6
F. nucleatum 12230 lam0.4 ± 0.00.1 ± 0.00.1 ± 0.0
F. nucleatum DUMC 29291.5 ± 0.10.2 ± 0.00.5 ± 0.0
F. nucleatum DUMC 33492.2 ± 0.52.0 ± 0.3ND
F. nucleatum DUMC 13562.3 ± 0.20.6 ± 0.1ND
F. nucleatum DUMC 20791.3 ± 0.10.4 ± 0.11.5 ± 0.6
F. gonidiaformans DUMC CF65-1<0.01<0.01<0.01
F. gonidiaformans DUMC CF63-1<0.01<0.01<0.01
E. coli DH5α<0.01<0.01ND
  • a Invasion and attachment were calculated as (no. of bacteria recovered after cell lysis) × 100/(total bacteria initially added). The values here are results from one representative experiment, shown as the average of duplicates ± standard deviation. Each experiment was repeated at least three times. The limit of detection was <0.01%. ND, not determined.