TABLE 3.

Whole-cell ELISA and bactericidal titers of mouse antibodies raised against rLP2086 tested against multiple serogroup B meningococcal strains

Test strainTest strain serosubtyperLP2086-8529 anti- sera (subfamily B)rLP2086-2996 anti- sera (subfamily A)
Whole-cell ELISA titeraBC50 titerbWhole-cell ELISA titeraBC50 titerb
Subfamily B
    539P1.7-2,3>1,458,0003,200
    H44/76P1.7,16>1,458,0003,20056,386<25
    H355P1.19,15>1,458,0003,200
    CDC-937P1.7-2,3-4>1,458,000>800
    M97 252097P1.7-2,16>1,458,000>800
    6940P1.18,25,6900,162>80011,157
    M982P1.22,9435,9092006,424
    880049P1.7-2,4349,91240010,588
    CDC-1573P1.7-1,1102,508257,871
Subfamily A
    870446P1.12-1,13389,829800>1,458,000>800
    M98 250771P1.22,14139,397<25447,867800
    M98 250732P1.22,14-123,928241,510>800
    M97 252697P1.18,25,699,580320,732>800
    6557P1.22-1,1425,590<2577,319<25
    NMBP1.5-1,2-2<2,000<258,979<25
  • a Endpoint titers expressed as the reciprocal of the dilution at absorbance = 0.1.

  • b Bactericidal (BC50) titers are represented as the reciprocal of the dilution of antiserum that reduced viable cell count by 50%. Week 0 normal mouse sera had BC50 titers of <25. A dash indicates samples were not tested.