TABLE 2.

Primers used in PCR amplification experiments

Primer nameSequenceRestriction site (underlined)
2086NDE5′-CTATTCTGCATATGACTAGGAGC-3′NdeI
3STP20865′-GCGCGGATCCTTACTGCTTGGCGGCAAGACC-3′BamHI
5UNI20865′-CTATTCTGCGTATGACTAG-3′NAa
3UNI20865′-GTCCGAACGGTAAATTATCGTG-3′NA
5INT20865′-TGCCGATGCACTAACCGCACC-3′NA
3INT20865′-CGTTTCGCAACCATCTTCCCG-3′NA
5OUT20865′-GACAGCCTGATAAACC-3′NA
3OUT20865′-GATGCCGATTTCGTGAAC-3′NA
N2086ILE5′-GCGCAGATCTCATATGAGCAGCGGAGGGGGTGGTGTCGCCGCCGAYATWGGTGCGGGGCTTGCCG-3′BglII/NdeI
20863STP5′-GAGATCTCACTCACTCATTACTGCTTGGCGGCAAGACCGATATG-3′BglII
N1573PCR5′-GAGATCTCATATGAGCAGCGGAGGCGGCGGAAGC-3′BglII
N29963STP5′-GAGATCTCACTCACTCACTACTGTTTGCCGGCGATGCCGATTTC-3′BglII
5LIP20865′-GCGGATCCAGCGGAGGGGGTGGTGTCGCC-3′BamHI
3SPH20865′-GCGCATGCTTACTGCTTGGCGGCAAGACCGATATG-3′SphI
5LIP15735′-GCGGATCCAGCGGAGGCGGCGGAAGC-3′BamHI
5LIP29965′-GCGGATCCAGCGGAGGCGGCGGTGTCGCC-3′BamHI
N2996SPH5′-GCGCATGCCTACTGTTTGCCGGCGATG-3′SphI
5LIP7715′-GCGGATCCAGCGGAAGCGGAAGCGGAGGCGGCGGTGTCGCC-3′BamHI
PorABg12Fwd5′-CGCGAGATCTCATATGGATGTCAGCCTATACGGCGAAATCAAAGCCGGCGTGGAAGGCAGGAACTACCAG-3′BglII/NdeI
PorABg12Ter5′-CGCGAGATCTTCAGTCACTCATTAGAATTTGTGGCGCAAACCGACG-3′BglII
  • a NA, not applicable.