TABLE 1.

Effect of uptake of different agents on infection of BMMφ or PMφ with C. burnetii phase II

Host cell treatmentaPMφbBMMφb
No addition8.0 ± 0.411.1 ± 1.2
Apo-heat14.7 ± 0.2**33.4 ± 7.4*
Apo-irrad14.5 ± 1.7*20.6 ± 2.5**
Fixed9.2 ± 1.315.1 ± 5.8
Lysed8.5 ± 0.213.4 ± 0.7
Live splenocytes9.3 ± 0.69.0 ± 0.5
AG13.8 ± 0.2**22.8 ± 0.9*
Apo-heat + AG15.2 ± 0.9*28.0 ± 5.6*
  • a Macrophages were treated 24 h prior to infection with three particles per cell or 1 mM AG.

  • b Percentage of cells (mean ± standard error) containing LRVs 6 days after C. burnetii infection. Significantly different result compared to control cells are indicated with asterisks (*, P < 0.05; **, P < 0.01).