TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaReference or source
H. ducreyi strains
    35000Wild-type strain isolated in Winnipeg, Manitoba, Canada26
    35000.1Isogenic lspA1 mutant; Kanr66
    35000.88Isogenic lspB mutant; ChlrThis study
    35000.188Isogenic lspA1 lspB mutant; Kanr ChlrThis study
    A77Serum-sensitive strain7
Plasmids
    pACYC184Cloning vector able to replicate in both E. coli and H. ducreyi; Chlr14
    pWKS30Low-copy cloning vector for E. coli64
    pCW156pRSETB (Invitrogen) with a 0.22-kb PCR product encoding amino acids 27 to 100 of LspB ligated into the BamHI and EcoRI sites; AmprThis study
    pCW158pWKS30 with the 2.88-kb PCR product containing the entire lspB gene ligated into the PstI and BamHI sites; AmprThis study
    pCW159pCW158 with the 0.7-kb SmaI fragment of pCWnpCAT1 (containing a nonpolar chloramphenicol resistance cartridge) ligated into the HpaI site; Chlr AmprThis study
    pCW1771.95-kb PvuII-NruI fragment of pACYC184 ligated to the 1.2-kb MamI-EcoRV fragment (kanamycin resistance gene) of pLS88; Kanr ChlrThis study
    pCW173pACYC184 with the 2.65-kb SalI-BamHI fragment containing the lspB gene ligated into the SalI and BamHI sites; ChlrThis study
    pCW2254.3-kb PvuII-NruI fragment of pCW173 ligated to the 1.2-kb MamI-EcoRV fragment (kanamycin resistance gene) of pLS88; KanrThis study
    pCWnpCAT10.7-kb PCR product containing a promoterless chloramphenicol resistance cartridge from pACYC184 ligated into the SmaI site of pBluescript II KS(+)This study
  • a Kan, kanamycin; Chl, chloramphenicol; Amp, ampicillin.