TABLE 2.

Lesion formation by wild-type and mutant H. ducreyi strains in the temperature-dependent rabbit modela

ExptStrainInoculum size (CFU)Lesion score (mean ± SD) on day:P valueb
247
1350001054.00 ± 0.004.00 ± 0.004.00 ± 0.00
35000.88 (lspB mutant)1053.00 ± 0.002.75 ± 0.492.71 ± 0.490.0001c
350001043.63 ± 0.533.63 ± 0.533.71 ± 0.49
35000.88 (lspB mutant)1043.00 ± 0.002.25 ± 0.491.86 ± 1.07
2350001054.00 ± 0.003.88 ± 0.353.88 ± 0.35
35000.88(pCW177)1053.00 ± 0.002.88 ± 0.352.63 ± 0.740.0003c
35000.88(pCW225)1053.13 ± 0.353.13 ± 0.353.25 ± 0.460.0001 (0.0088)c,d
350001043.13 ± 0.353.13 ± 0.643.00 ± 0.93
35000.88(pCW177)1042.88 ± 0.352.00 ± 0.531.63 ± 0.52
35000.88(pCW225)1043.00 ± 0.002.25 ± 0.462.38 ± 0.74
  • a Seven rabbits were used in experiment 1, and eight rabbits were used in experiment 2.

  • b P value calculated for the difference between wild-type and test strain lesion scores for the three scoring days by using both inoculum-sizes.

  • c Significant difference.

  • d The P value of 0.0088 refers to the difference between the 35000.88(pCW177) and 35000.88(pCW225) strains.