TABLE 1.

Matrix of HMM scores for sortase subfamilies

Sortase subfamilyaHMM score for sortase subfamilyb
SrtASrtB345Gram negative
SrtA150-610c−110d−79−56−56−146
SrtB−155548-674−215−141−182−176
3−10−146150-547−42−68−87
4−103−158−72281-460−35−55
5−72−163−1437380-672−87
Gram negative−134−184−154−13−95347-630
• a Six subfamilies of sortases homologs as clustered by sequence homology. The sortase subfamilies SrtA, SrtB, 3, 4, 5, and gram-negative contain 42, 17, 54, 13, 14, and 15 homologs, respectively.

• b A HMM for each sortase subfamily was constructed using HMMER. A HMM score was calculated for every sortase homolog to each subfamily HMM and is indicative of the similarity between a homolog and the consensus sequence of the given sortase subfamily (the higher the score is, the more similar a sortase homolog is to a subfamily HMM).

• c The range of HMM scores (in boldface) exhibited by sortase homologs within a subfamily to the HMM of the same subfamily. The range of scores is indicative of the similarity of these sortase homologs to the consensus sequence and hence to one another.

• d One score is given when a comparison is made between a set of sortase homologs within a subfamily to the HMM of a different subfamily. The score is the highest score that a sortase homolog within a given subfamily has to the HMM of a different subfamily.