TABLE 4.

Genomic clusters of sortases and CWS-containing proteins

SpeciesSortase subfamilies in genomic clusteraDistribution of sortases and CWS-substrates in genomic clusterb
SortaseSubstratec1 sortase2 sortases3 sortases
1 sub.2 sub.3 sub.4 sub.1 sub.2 sub.3 sub.0 sub.3 sub.
Actinobacteria (high-G+C gram-positive bacteria)
    Corynebacterium diphtheriae33/5d
33/5
33
    Corynebacterium efficiens33/5
33/5
    Bifidobacterium longum DJ010A33
    Bifidobacterium longum NCC270533
Firmicutes (gram-positive bacteria)
    Bacillus
        Bacillus anthracis A2012BB
44
        Bacillus anthracis AmesBB
44
        Bacillus anthracis KrugerBBB
44
        Bacillus anthracis Western NABB
44
        Bacillus cereus ATCC 1098733
33
BB
44
33
        Bacillus cereus ATCC 14579BB
44
33
        Bacillus haloduransXeX
XX
XX
BB
44
        Bacillus subtilis44
        Geobacillus stearothermophilus44
        Oceanobacillus iheyensis44
    Listeria
        Listeria innocuaBB/A
        Listeria monocytogenes 4bBB/A
        Listeria monocytogenes EGD-eBB/A
    Staphylococcus
        Staphylococcus aureus COLBB/A
        Staphylococcus aureus MRSA252BB/A
        Staphylococcus aureus MSSA476BB/A
        Staphylococcus aureus MW2BB/A
        Staphylococcus aureus Mu50BB/A
        Staphylococcus aureus N315BB/A
        Staphylococcus aureus NCTC 8325BB/A
    Enterococcus
        Enterococcus faecalis33
XX
        Enterococcus faecium33
33
33
XX
    Leuconostocaceae
        Leuconostoc mesenteroides33
    Streptococcaceae
        Lactococcus lactis33
        Streptococcus agalactiae 2603V/R33
33
        Streptococcus agalactiae A90933
33
        Streptococcus agalactiae NEM31633
33
        Streptococcus equi33
        Streptococcus pneumoniae 670-6B33
        Streptococcus pneumoniae TIGR433
        Streptococcus pyogenes M1 GASX/3X
        Streptococcus pyogenes MGAS315XX
        Streptococcus pyogenes MGAS8232XX
        Streptococcus pyogenes ManfredoXX
        Streptococcus pyogenes SSI-IXX
        Streptococcus suis33/A
    Clostridia
        Clostridium acetobutylicumXX
        Clostridium botulinumXX
        Clostridium perfringens ATCC 1312433
XX
        Clostridium perfringens 1333
XX
        Clostridium tetaniXX
        Ruminococcus albus33
Proteobacteria (purple bacteria and relatives—gram-negative)
    Bradyrhicobium japonicum66
    Colwellia psychroerythraea66
    Microbulbifer degradans66
    Shewanella oneidensis66
    Shewanella putrefaciens66
Total no. of clusters311018213813
  • a Sortase homologs are clustered into subfamilies according to sequence homology using BLAST profiles and HMMs.

  • b Numbers of sortase homologs and CWS-containing proteins that are genomically adjacent in a “genomic cluster” are given in column heads. sub., substrate(s).

  • c The subfamily of the sortase(s) and the CWS-containing protein(s) in the genomic cluster.

  • d Shill indicates that genomic cluster encodes sortases and/or CWS-containing proteins from more than one sortase subfamily.

  • e Sortase homolog(s) in the genomic cluster is not readily classified into a subfamily based on sequence homology and therefore is designated as belonging to “subfamily X.” There are 15 genomic clusters of these unclassified sortase homologs and CWS-containing proteins.