Reactivities of MAb and polyclonal sera to M. leprae and M. tuberculosis whole CFP-10 and homologous peptides

M. leprae CFP-10M. tuberculosis CFP-10p1p2p3p4p5p6p7p8p9p10p11p12p13p14p15p16p17p18
Mouse anti-M. leprae CFP-10+++b+/−++++/−++++++++/−+++++/−+++++
Rabbit anti-M. leprae CFP-10++++++++/−+++++++++++/−+++++++
Mouse anti-M. tuber- culosis CFP-10+/−++++/−+++++++++/−+/−+++++++
  • a 3A10.2C8, mouse MAb specific for M. leprae CFP-10.

  • b +++, optical density as determined by ELISA, >4.00; ++, optical density, 1.50 to 4.00; +, optical density, 0.50 to 1.49; +/−, optical density, 0.20 to 0.49; −, background reading (optical density, <0.20). The polyclonal sera were tested at a 1:500 dilution, and the MAb was tested at a 1:2 dilution. There was no response of either polyclonal antisera or the MAb to any heterologous peptide.