TABLE 1.

Strains and plasmids used in this work

Strain or plasmidCharacteristicsReference or source
V. cholerae
    O395Wild-type classical biotype with lacZ deletion; SmrM. Waldor (31)
    O395, toxR::pVM55toxR insertion mutant; Smr AprM. Waldor (32)
    N16961Wild-type El Tor biotypeM. Waldor (17)
    N16961, toxR::pVM55toxR insertion mutant; Smr AprThis study
    O395, bipA::pJM1bipA insertion mutant; Smr AprThis study
    N16961, bipA::pJM1bipA insertion mutant; Smr AprThis study
    O395, VJ740toxT internal deletion mutant; SmrV. DiRita (10)
    O395, KKV780ompU deletion mutant; SmrK. Klose (37, 38)
    O395, KKV809ompT deletion mutant; SmrK. Klose (37, 38)
    O395, KKV884ompU/ompT double mutant; SmrK. Klose (38)
    O395, tolA::pGP704tolA insertion mutant; Smr AprD. Heilpern (18)
    O395, tolB::pGP704tolB insertion mutant; Smr AprD. Heilpern (18)
    O395, tolC::pGP704tolB insertion mutant; Smr AprThis study
pKEK253pU-ompU; AprK. Klose (37, 38)
pKEK255pT-ompT; AprK. Klose (37, 38)
pKEK257pU-ompT; AprK. Klose (37, 38)
pBAD28Apr CmrInvitrogen
pVM55Apr; pGP704 derivative with internal fragment of toxRV. Miller (32)
pJM1Apr; pGP704 derivative with internal fragment of bipAThis study