TABLE 2.

LOS glycoforms observed in plate-grown, planktonic, and biofilm NTHI strain 2019

GlycoformProposed compositionaCalculated [M-H] withb:
Neu5AcHexNAcHexHepKdo(P)ChoPOne PEATwo PEAThree PEA
Asialo glycoforms
    A1312,113.82,236.92,360.0
    B2312,276.02,399.02,522.1
    Bc23112,441.12,564.22,687.2
    C3312,438.12,561.22,684.2
    D4312,600.32,723.32,846.4
    E5312,762.42,885.53,008.5
    F13312,641.32,764.4
    G14312,803.52,926.5
    H15312,965.63,088.7
Sialylated glycoformsd
    B*12312,690.32,813.4
    B**22312,858.52,981.63,104.6
    D*14312,891.53,014.6
    D**24313,182.83,305.8
    E*15313,053.73,176.73,299.8
    E**25313,344.93,468.03,591.0
    F*113313,055.63,178.7
    H*115313,256.93,379.93,503.0
    I*116313,542.13,665.1
    I**216313,710.33,833.33,956.4
    I***316314,001.54,124.6
  • a All components contain the O-deacylated lipid A moiety. Kdo(P), 3-deoxy-d-manno-octulosonic acid phosphate; ChoP, phosphorylcholine; Neu5Ac, N-acetylneuraminic acid; HexNAc, N-acetylhexosamine; Hex, hexose; Hep, heptose.

  • b All masses are average values. PEA, phosphoethanolamine.

  • c The glycoform contains a phosphorylcholine moiety.

  • d The numbers of asterisks indicate the numbers of Neu5Ac residues.