TABLE 1.

Strains and plasmids used in this study

Strain or plasmidReferenceCommentsa
S. epidermidis
    145750Isolate from infected central venous catheter
    M1536rsbU-Tn917 mutant of S. epidermidis 1457 used as recipient by electroporation
    1457rsbUThis studyrsbU::erm derivate from S. epidermidis 1457 derived by allelic gene replacement
    1457rsbVThis studyrsbV::erm derivate from S. epidermidis 1457 derived by allelic gene replacement
    1457rsbWThis studyrsbW::erm derivate from S. epidermidis 1457 derived by allelic gene replacement
    1457sigBThis studysigB::erm derivate from S. epidermidis 1457 derived by allelic gene replacement
    1457rsbUVWThis studyrsbUVW::erm derivate from S. epidermidis 1457 derived by allelic gene replacement
    1457rsbUVWsigBThis studyrsbUVWsigB::erm derivate from S. epidermidis 1457 derived by allelic gene replacement
    840050Blood culture isolate
    8400-M1536rsbU-Tn917 mutant of S. epidermidis 8400 derived by phage transduction
    8400rsbUThis studyrsbU::erm derivate from S. epidermidis 8400 derived by phage transduction
    8400rsbVThis studyrsbV::erm derivate from S. epidermidis 8400 derived by phage transduction
    8400rsbWThis studyrsbW::erm derivate from S. epidermidis 8400 derived by phage transduction
    8400sigBThis studysigB::erm derivate from S. epidermidis 8400 derived by phage transduction
    8400rsbUVWThis studyrsbUVW::erm derivate from S. epidermidis 8400 derived by phage transduction
    8400rsbUVWsigBThis studyrsbUVWsigB::erm derivate from S. epidermidis 8400 derived by phage transduction
E. coli
    TOP 10Invitrogen
    SJrsbUThis studyTOP 10 containing pSJrsbU
    SJrsbVThis studyTOP 10 containing pSJrsbV
    SJrsbWThis studyTOP 10 containing pSJrsbW
    SJsigBThis studyTOP 10 containing pSJsigB
    SJrsbUVWThis studyTOP 10 containing pSJrsbUVW
    SJrsbUVWsigBThis studyTOP 10 containing pSJrsbUVWsigB
Plasmids
    pCRII TOPOInvitrogenE. coli cloning vector for direct cloning of PCR fragments
    pBT210ts E. coli/Staphylococcus shuttle vector
    pSJrsbUThis studyts vector for allelic gene replacement of rsbU in S. epidermidis
    pSJrsbVThis studyts vector for allelic gene replacement of rsbV in S. epidermidis
    pSJrsbWThis studyts vector for allelic gene replacement of rsbW in S. epidermidis
    pSJsigBThis studyts vector for allelic gene replacement of sigB in S. epidermidis
    pSJrsbUVWThis studyts vector for allelic gene replacement of rsbUVW in S. epidermidis
    pSJrsbUVWsigBThis studyts vector for allelic gene replacement of rsbUVWsigB in S. epidermidis
  • a ts, temperature sensitive.